| Business | Phone |
| Foxmeadows Daycare 3227 Old Jennings Rd, Middleburg 32068 | (904) 282-7050 |
| Kids World Child Care Center 5390 County Road 218, Middleburg 32068 | (904) 291-1500 |
| La Petite Academy 3150 County Road 220, Middleburg 32068 | (904) 282-5225 |
| Opk South 3425 Habitat Dr, Middleburg 32068 | (904) 282-9310 |
| Tender Hearts Day Care 29 Cinnamon St, Middleburg 32068 | (904) 282-9390 |
| Wee-Care Pre School 4717 State Road 218, Middleburg 32068 | (904) 282-7320 |