| Business | Phone |
| A Church Of Christ Inc 1633 Lisa Dawn Dr, Middleburg 32068 | (904) 291-1711 |
| Apostolic Lighthouse 5040 County Road 218, Middleburg 32068 | (904) 282-3095 |
| Black Creek Church Of Christ 3216 State Road 218, Middleburg 32068 | (904) 282-4033 |
| Black Pond Baptist Church 3644 Old Jennings Rd, Middleburg 32068 | (904) 282-5718 |
| Brannanfield Baptist Church 908 Branan Field Rd, Middleburg 32068 | (904) 282-7970 |
| Calvary Baptist Church 1532 Long Bay Rd, Middleburg 32068 | (904) 282-0407 |
| Church Of Jesus Christ Of Lds 4342 State Road 218, Middleburg 32068 | (904) 282-1040 |
| Church Of The Brethren 1651 Russell Rd, Middleburg 32068 | (904) 282-5048 |
| Cinnamon Street Baptist Church 20 Cinnamon St, Middleburg 32068 | (904) 282-0881 |
| Clay Hill Church Of God 5075 County Road 218, Middleburg 32068 | (904) 291-9272 |
| Embassy Of Christ 2713 County Road 220, Middleburg 32068 | (904) 276-3327 |
| Faith Wesleyan Church 582 Plantation Dr, Middleburg 32068 | (904) 272-1754 |
| Family Of God Po Box 854, Middleburg 32050 | (904) 491-4910 |
| First Assembly Of God 3167 County Road 215, Middleburg 32068 | (904) 282-1466 |
| First Baptist Church 2645 Blanding Blvd, Middleburg 32068 | (904) 282-5289 |
| First Church Of God 3965 Old Jennings Rd, Middleburg 32068 | (904) 282-1810 |
| Lakeside Community Church 564 Tara Farms Dr, Middleburg 32068 | (904) 272-3302 |
| Living Waters Of Middleburg 4524 Camellia St, Middleburg 32068 | (904) 291-0704 |
| Madeira Baptist Church 1650 Blanding Blvd, Middleburg 32068 | (904) 291-1880 |
| Middleburg Church Of Christ 3155 County Road 215, Middleburg 32068 | (904) 282-5616 |
| Middleburg Church Of God 17 Azalea Ave, Middleburg 32068 | (904) 282-2957 |
| Middleburg Presbyterian Chr 4564 Rosemary St, Middleburg 32068 | (904) 282-0130 |
| Middleburg United Methodist 3925 Main St, Middleburg 32068 | (904) 282-5589 |
| Morningstar Family Church 3900 Main St, Middleburg 32068 | (904) 282-3393 |
| Oak Grove Baptist Church County Road 215, Middleburg 32068 | (904) 282-2984 |
| Pinewood Presbyterian Church 198 Knight Boxx Rd, Middleburg 32068 | (904) 272-7177 |
| Ronvine Building 3108 County Road 220, Middleburg 32068 | (904) 291-6441 |
| St Lukes Catholic Church 1606 Blanding Blvd, Middleburg 32068 | (904) 282-0439 |
| St Mark Missonary Baptist Chr 2703 Forman Cir, Middleburg 32068 | (904) 291-3700 |
| St Peter's Lutheran Church 1614 Blanding Blvd, Middleburg 32068 | (904) 282-8876 |